![]() Among these, hydrogels are becoming increasingly attractive due to their high quantity of water and biocompatibility, while their mechanical and structural properties mimic many soft tissues 1. In TE, various types of biomaterials are used as scaffolds. Tissue engineering (TE) is a fast-growing field that aims to restore the structure and function of diseased or damaged tissue through the use of cells, supportive biomaterials, and biologically active molecules 1. Our results show that OPT has a sensitivity to assess in real 3D cultures the differences of cellular responses to the properties of biomaterials supporting the cells. We observed that an elongated morphology of cells, thus good material response, in gelatin-GG and Geltrex hydrogels compared with basic GG. Our results showed the usability of the method to quantify the cellular responses to biomaterial environment. We investigated cell morphology, density, distribution, and viability in 3D living cells. Using our tools, we demonstrated the method by analyzing cell response in three different hydrogel formulations in 3D with 1.5 mm diameter samples of: gellan gum (GG), gelatin functionalized gellan gum (gelatin-GG), and Geltrex. To address the problem, we have developed a new protocol using Optical Projection Tomography (OPT) to extract quantitative and qualitative measurements from hydrogel cell cultures. This is difficult using established optical microscopy techniques such as wide-field or confocal microscopy. ![]() In TE, there is an urgent need for methods to image actual three-dimensional (3D) cell cultures and access the living cells. Assessing cell morphology and function, as well as biomaterial performance in cell cultures, is one of the key challenges in cell biology and tissue engineering (TE) research. ![]()
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